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qdot 655  (Quantum Dot Inc)

 
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    Structured Review

    Quantum Dot Inc qdot 655
    Qdot 655, supplied by Quantum Dot Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qdot 655/product/Quantum Dot Inc
    Average 90 stars, based on 1 article reviews
    qdot 655 - by Bioz Stars, 2026-03
    90/100 stars

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    Image Search Results


    The tissue was successively stained for GFAP-positive astrocytes (QDot 585, yellow) (a), MBP-positive myelin (QDot 655, red) (b), Hoechst 34580 (blue) (c) and imaged using a 4X (0.13NA) and 40x water dipping objective (0.8NA) (e-g) respectively. Triple stained tissue (d) with 4X (0.13NA) and 40x water dipping objective (0.8NA) (h). Post-expansion (i-l) images of GFAP-positive astrocytes (QDot 585, yellow) (i), MBP-positive myelin (QDot 655, red) (j), Hoechst (blue) (k) and triple imaging (l) using the same 40x objective shows the preservation of all labels. The scale bar represents 50 μ m (physical size) to allow comparison with the pre-expansion image (h). However, expansion microscopy physically magnified the sample ≈ 4.1X, so the 50 μ m scale represents ≈ 12.2 μ m structurally.

    Journal: PLOS One

    Article Title: Fast photostable expansion microscopy using QDots and deconvolution

    doi: 10.1371/journal.pone.0325155

    Figure Lengend Snippet: The tissue was successively stained for GFAP-positive astrocytes (QDot 585, yellow) (a), MBP-positive myelin (QDot 655, red) (b), Hoechst 34580 (blue) (c) and imaged using a 4X (0.13NA) and 40x water dipping objective (0.8NA) (e-g) respectively. Triple stained tissue (d) with 4X (0.13NA) and 40x water dipping objective (0.8NA) (h). Post-expansion (i-l) images of GFAP-positive astrocytes (QDot 585, yellow) (i), MBP-positive myelin (QDot 655, red) (j), Hoechst (blue) (k) and triple imaging (l) using the same 40x objective shows the preservation of all labels. The scale bar represents 50 μ m (physical size) to allow comparison with the pre-expansion image (h). However, expansion microscopy physically magnified the sample ≈ 4.1X, so the 50 μ m scale represents ≈ 12.2 μ m structurally.

    Article Snippet: Mouse hippocampus was labeled with GFAP (Qdot 585) , MBP (Qdot 655) , Hoechst 34580 ( ) as a nuclear counterstain, and an overlay of the three stains ( ).

    Techniques: Staining, Imaging, Preserving, Comparison, Microscopy

    Both labels target MBP. Timelapse measurements were taken for 35 min at 10 s intervals using a 40x water immersion objective (0.8NA). (a) The normalized intensity is plotted over time. Low-magnification images of Alexa 594 samples were obtained at 10X (0.45NA) both before (b) and after (c) 30 minutes of exposure showing the bleached region (arrows) (scale bar 200 μ m). Alexa 594 time points were taken at 0 min (d), 10 min (e), 15 min (f), and QDot 655 time points were taken at 0 min (g), 30 min (h), and 50 min (i) (scale bar 50 μ m).

    Journal: PLOS One

    Article Title: Fast photostable expansion microscopy using QDots and deconvolution

    doi: 10.1371/journal.pone.0325155

    Figure Lengend Snippet: Both labels target MBP. Timelapse measurements were taken for 35 min at 10 s intervals using a 40x water immersion objective (0.8NA). (a) The normalized intensity is plotted over time. Low-magnification images of Alexa 594 samples were obtained at 10X (0.45NA) both before (b) and after (c) 30 minutes of exposure showing the bleached region (arrows) (scale bar 200 μ m). Alexa 594 time points were taken at 0 min (d), 10 min (e), 15 min (f), and QDot 655 time points were taken at 0 min (g), 30 min (h), and 50 min (i) (scale bar 50 μ m).

    Article Snippet: Mouse hippocampus was labeled with GFAP (Qdot 585) , MBP (Qdot 655) , Hoechst 34580 ( ) as a nuclear counterstain, and an overlay of the three stains ( ).

    Techniques: